To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In
Cell Cycle Synchronization: Methods and Prools
, experts in the field supply detailed prools providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful
Methods in Molecular Biology™
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Detailed and easy to follow,
is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested prools designed for doing so.