The laboratory technique which deals with the separation of a mixture is known as chromatography. This technique involves the dissolution of the mixture in a fluid which is known as the mobile phase. It is then introduced to another material which is known as the stationary phase. Separation occurs when various constituents of the mixture travel at different speeds. Chromatography is mainly classified into two types, preparative and analytical. Preparative chromatography is aimed at separating the constituents of the mixture for later use. It can also be considered as a form of purification. Analytical chromatography generally makes use of lesser amounts of material and is usually used to measure the relative quantity of analytes. This book is compiled in such a manner, that it will provide in-depth knowledge about the theory and practice of chromatography. It will serve as a valuable source of reference for graduate and post graduate students. This book is an essential guide for both academicians and those who wish to pursue this discipline further.