The field of biotechnology has witnessed transformative advancements over the past few decades, with the CRISPR-Cas9 system standing out as one of the most groundbreaking developments. This powerful genome-editing tool has revolutionized the way scientists approach genetic modifications, enabling precise, efficient, and versatile editing of the DNA in living organisms. As we delve into the realm of vaccine development, CRISPR-Cas9 emerges as a pivotal technology, offering unprecedented opportunities to design next-generation recombinant vaccines that are more effective, targeted, and adaptable to a wide range of diseases. "CRISPR-Cas9 Mediated Next Generation Recombinant Vaccine Development" explores the convergence of CRISPR technology with vaccinology, presenting a comprehensive overview of how this innovative tool is reshaping the landscape of vaccine research and development. This book is a collective effort to compile the latest research, methodologies, and applications of CRISPR-Cas9 in creating recombinant vaccines that could potentially change the course of infectious disease management.