An entrepreneurial ecosystem is characterized by the structure and interactions of organizations, firms, institutions, and individuals in a specific locale that is conducive to entrepreneurship. It can be defined as a set of interdependent actors and factors that are governed in such a way that they enable productive entrepreneurship in a particular territory. The World Bank has developed a new toolkit, Developing Entrepreneurial Ecosystems for Digital Businesses and Beyond, for entrepreneurial ecosystem assessments, including dedicated methodologies and data sets, to nurture digital entrepreneurship. This toolkit builds on the World Bank’s expertise in collecting and analyzing firm-level data, assessing the quality and efficiency of policies that support innovation and small and medium enterprises, and deriving insights from spatial economics to inform subnational analysis. The toolkit consists of six modules: cross-country context analysis, assessing local entrepreneurial ecosystems, digital entrepreneurship and tech start-ups, mapping public programs and intermediary organizations, digital market regulations, and policy options to support entrepreneurial ecosystems.