Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Prools provides a diverse collection of readily reproducible cellular and molecular prools for the manipulation of nonhuman embryonic stem cells. Volume one, Embryonic Stem Cell Prools: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular prools for the isolation, maintenance, and characterization of embryonic stem cells. The second volume, Embryonic Stem Cell Prools: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.