An elucidative account based on the sustainable degradation of lignocellulosic biomass has been described in this book. It covers important aspects of sustainable degradation of lignocellulosic biomass which plays a crucial role in the economic production of numerous value-added products and biofuels which are safe to the environment. Enzymatic hydrolysis process and various pretreatment methods along with the depiction of cell wall components have been elucidated broadly. This book provides in-depth analysis regarding the enhancement in methodologies for the biomass pretreatment, the analytical procedures for biomass characterization, hemicellulose and cellulose breakdown into fermentable sugars and bioconversion of cellulosics into biofuels. Mechanistic analysis of biomass pretreatment and enzymatic hydrolysis has also been elucidated, focusing on the key factors responsible for these processes at industrial scale.