This research work on Job Stress and its Influence on the Performance of Nursing Personnel Working at the Territorial Military Hospital in the city of Guayaquil, from October 2015 to February 2016, had the objective of determining levels of job stress in nursing personnel and its influence on job performance. The population consisted of 15 nursing graduates and 6 nursing assistants. A descriptive study was carried out. The instrument used was a questionnaire. The results show that 54% of the personnel surveyed were overworked; 73% of the personnel identified that they were understaffed in the internal medicine area, which generates work overload in the few personnel on duty and that they must redouble their efforts to cover these gaps; likewise, 56% of the personnel experienced a low level of concentration, with omissions and forgetfulness in the fulfillment of tasks; 53% showed a lackadaisical treatment of colleagues or patients. It is worth noting that 50% of the personnel show attitudes of lack of motivation and lack of interest in collaborative work.