This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through
in vitro
growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
Mouse Oocyte Development: Methods and Prools
aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.