This volume describes prools for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the
Methods in Molecular Biology
series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory prools, and tips on troubleshooting common problems and avoiding pitfalls.
Authoritative and practical,
Peptidomics:
Methods and Strategies
provides useful guidance for studies in the rapidly growing field of peptidomics.