In this highly anticipated update of the extremely successful Protein Targeting Prools, experts from around the world provide the latest prools on for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Unlike the first edition, however, this volume places emphasis on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems.
Authors provide state-of-the-art information on the fast moving fields of import machinery of mihondria and plastids, as well as a detailed prool on the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching. Bacterial protein targeting prools using the Sec-system, type-V secretion apparatus, and the Tat-pathway are described, as is a periplasmic targeting prool. Chapters also include bioinformatics methods to guide readers through the ever-increasing number of in silico tools currently available. Editor Mark van der Giezen has succeeded in including targeting prools from different systems, including animal, plant, fungal, and protist models, providing insights into the intricate challenges presented by these diverse systems.
Authoritative and state-of-the-art, Protein Targeting Prools, Second Edition, provides useful techniques that will aid scientists working with a variety of organisms.