This detailed volume explores techniques to study reactive oxygen species (ROS) in plants and to characterize their roles in development and stress responses. Beginning with a section on strategies to induce ROS production, the book continues with methods to visualize ROS and detect changes in redox homeostasis, small-scale and targeted analyses for investigating the effects of ROS accumulation during stress on plant physiology and metabolism, as well as systems biology approaches to understand ROS functions. Written for the highly successful series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, serves as a vital resource that any researcher, and in particular young researchers, can use and adapt to further our knowledge of this dynamic area of plant science.