This volume provides prools and procedures for determining and modeling RNA structure. Chapters guide the reader through prools for RNA secondary structure prediction, single sequence modeling, Crumple, RNAstructure to model conserved secondary structures with multiple homologs, the prediction of bimolecular secondary structures with RNAstructure, STarMir, prools for structure mapping, mapping data to constrain or restrain RNA secondary structure prediction with RNAstructure, unassigned NMR resonances, modeling prools for Rosetta FARFAR, RNAComposer , ModeRNA, and MC-Fold. Written in the highly successful
Methods in Molecular Biology
series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and Practical,
RNA Structure Determination:Methods and Prools
aims to ensure successful results in the further study of this vital field.