Diagnosis of toxoplasmosis in pregnant women is mandatory and based mainly on serology. The aim of this study was to compare three serological techniques for the detection of anti-toxoplasmic IgG. Our study was carried out between January and April 2023, collecting 53 sera from pregnant women tested by the following techniques: ECLIA (Elecsys(R) Toxo IgG), quantitative ELISA (PlateliaTM Biorad), semi-quantitative ELISA (PlateliaTMTestline) and Blot-line Toxoplasma IgG. The average age of our patients was 29 ± 3.9 years. Serologies were requested during their first pregnancies (72%), in primiparous women (51%) and in 67% during the first trimester. ECLIA and quantitative and semi-quantitative ELISA showed complete agreement (p=1), but IgG titres by ECLIA were higher. On the other hand, almost perfect agreement (P= 0.947) was found when comparing ECLIA and Western Blot. ECLIA appears to be the most sensitive of the techniques studied. In addition, for titres close to the ECLIA positivity threshold, the use of a second confirmatory technique is recommended.